Freeze FracturingPreparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.
MicroscopyThe use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
VacuumA space in which the pressure is far below atmospheric pressure so that the remaining gases do not affect processes being carried on in the space.
CarbonA nonmetallic element with atomic symbol C, atomic number 6, and atomic weight 12.011. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE.
PlatinumPlatinum. A heavy, soft, whitish metal, resembling tin, atomic number 78, atomic weight 195.09, symbol Pt. (From Dorland, 28th ed) It is used in manufacturing equipment for laboratory and industrial use. It occurs as a black powder (platinum black) and as a spongy substance (spongy platinum) and may have been known in Pliny's time as "alutiae".
CarbonA nonmetallic element with atomic symbol C, atomic number 6, and atomic weight 12.011. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE.
CarbonA nonmetallic element with atomic symbol C, atomic number 6, and atomic weight 12.011. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE.
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