Freeze EtchingA replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces.
CellsThe fundamental, structural, and functional units or subunits of living organisms. They are composed of CYTOPLASM containing various ORGANELLES and a CELL MEMBRANE boundary.
TemperatureNo Entry for Temperature
CellsThe fundamental, structural, and functional units or subunits of living organisms. They are composed of CYTOPLASM containing various ORGANELLES and a CELL MEMBRANE boundary.
SublimationA defense mechanism through which unacceptable impulses and instinctive urges are diverted into personally and socially acceptable channels; e.g., aggression may be diverted through sports activities.
WaterWater. A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
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